Last edited by Makazahn
Friday, November 20, 2020 | History

4 edition of Ion-Sensitive Intracellular Microelectrodes found in the catalog.

Ion-Sensitive Intracellular Microelectrodes

Roger Christopher Thomas

Ion-Sensitive Intracellular Microelectrodes

How to Make and Use Them (Biological techniques series)

by Roger Christopher Thomas

  • 388 Want to read
  • 8 Currently reading

Published by Academic Pr .
Written in English

    Subjects:
  • Electrodes, Ion selective,
  • Microelectrodes,
  • Technique,
  • Cytology

  • The Physical Object
    FormatHardcover
    Number of Pages123
    ID Numbers
    Open LibraryOL9283874M
    ISBN 100126887500
    ISBN 109780126887501

    Results obtained with ion-sensitive microelectrodes. Experimental studies on frog skeletal muscle of Hill [22], Fenn [19] and Gersh [20] provided indirect evidence for the view that virtually all water and K +ions exist in the free state in living cells. With the de-velopment of ion sensitive microelectrodes it was expected that free and bound Cited by: 3.   Consequently, ion-sensitive microelectrodes, absorbance pH indicators, fluorescence indicators and determination of brain pH by nuclear magnetic resonance (NMR) are covered. These chapters cover in fair detail the background and application of these various by: 1. The intracellular space has a volume of 80% (). The cerebrospinal fluid (CSF) volume rep- resents -lo% of brain weight (31). A. Methodology: Ion-Sensitive Microelectrode Much of the data described in this review stems from experiments with ion-sensitive microelectrodes (ISM). The intracellular K+, Na+, and Ca2+ of mechanosensory neurons in the central nervous system of the leech Hirudo medicinalis was measured using double-barreled ion-sensitive microelectrodes. 2. After inhibition of the Na(+)-K+ pump with 5 x 10(-4) M ouabain, the intracellular K+ activity (aKi) decreased, while the intracellular Na+ activity Cited by:


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Ion-Sensitive Intracellular Microelectrodes by Roger Christopher Thomas Download PDF EPUB FB2

This must have many consequences to my thoughts on experiments with microelectrodes. In this book, my concern is fo­ cussing on the description of an intracellular method that should lead to reliable in­ formation on cellular parameters. The methodical basis for any meaningful applica­ tion is Cited by: at a cellular level.

Ion-sensitive microelectrodes provide a means of directly assessing the extracellular or intracellular activity of an ion and for making prolonged measurements of these. It is the activity of an ion which is important because it, rather than the total or free concentration, determines, for example, the membrane potential,File Size: KB.

The Symposium on "Ion Selective Microelectrodes and Their Use in Excitable Tissues" was held in Prague from JulyIt was organized by the Institute of Physiology of the Czechoslovak Academy of Sciences as a satellite symposium of the XXVIII Interna­ tional Congress of Physiological Sciences in : Paperback.

Full text Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (K), or click on a page image below to browse page by page. Ion-sensitive microelectrodes provide a means of directly assessing the extracellular or intracellular activity of an ion and for making prolonged measurements of these.

It is the activity of an ion which is important because it, rather than the total or free concentration, determines, for example, the membrane potential, equilibrium potentials, or the thermodynamic conditions for ion transport mechanisms.

Microelectrode Methods for Intracellular Recording (Biological Techniques Series): Economics Books @ 5/5(1). For H + injection microelectrodes were pulled from quartz tubing filled with 1 M HCl and left with tips intact. Intracellular CaSMs were made from quartz glass and pHSMs from borosilicate glass [30, 31].

For surface pH, both liquid ion sensor and Hinke-style glass pH microelectrodes [32] were used. The pH-sensitive microelectrodes responded with 55 to 60 mV (at 35 °C) to a unit change of the pH in the bathing solution, the response being more than 90% complete within 30 to s.

The response times of the ion-sensitive microelectrodes used in our experiments were much faster than the changes in the intracellular ion activities : Joachim W. Deitmer, David Ellis. Performance of quartz ion-selective microelec- trodes Apart from their greater mechanical strength, which means that they penetrate tough connective tissue, do not become blunt, and can be wiped quite vigorously between the fingers, quartz ion- 63 selective microelectrodes for intracellular measure- ments, made as described, appear to function longer than those made of Pyrex by the method of Cited by: the use of ion-sensitive electrodes or ionophoresis of drugs.

Making microelectrodes One definition of a microelectrode (ME) might be: ‘an electrode constructed with a tip having the dimensions of the order of a micrometre (1 µm)’. Usually this means a glass micropipette of the type pioneered by Ling & Gerard (), which is filledFile Size: KB.

Emerging techniques: Ion-sensitive microelectrodes for intracellular use. TIBS 5, (). Reversal of the pHi-regulating system in a snail neuron. In: Current Topics in Membranes and Transport, 13, pp.ed.

E.L. Boulpaep. Regulation of intracellular Na+, K+ and H+ concentrations in snail neurons. Construction of these ion-sensitive microelectrodes for intracellular use remains an art, since all of the alternative fabrication procedures have not yet been explored.

TF~is article will review the construction and use of these Ca +-sensitive microelectrodes according to the methods currently used in our labs, Cited by:   Part of the Neuromethods book series (NM, volume 43) Ca 2+ -sensitive microelectrodes are time-consuming to make and require large robust cells.

But, they do not add to buffering and do not require expensive equipment. I describe how to make and use the electrodes and briefly consider the leakage by: 2. This website uses cookies to ensure you get the best experience on our website. Learn moreAuthor: Jeffrey L. Noebels.

The Yale Journal of Biology and Medicine [01 Sep52(5)]Author: JL Noebels. Theory, Potential and Existing State of Development.- 1.

Perspective: Ion-Selective Microelectrodes: Their Potential in the Study of Living Matter In Vivo.- 2. Ion-Selective Electrode Response in Biologic Fluids.- 3. Glass Microelectrodes for pH.- 4. Some Problems with an Intracellular PO2 Electrode.- 5.

Some Problems with the Antimony. A recipe for making double-barrelled ion-sensitive microelectrodes is given. Introduction The activities of a number of different ionic species can now be measured in biological tissues with ion-sensitive microelectrodes of the type in which a column of liquid sensor is lodged in the open tip of a glass micropipette (see e.g.

Ammann et al., ).Cited by: The measurement of intracellular ionic activity is crucial to a quantitative understanding of neuronal function. Intracellular ion-sensitive microelectrodes (ISMs) can now be routinely made which are capable of determining the ionic activities of Na +, K +, Cl −, H + and Ca 2+ and of monitoring their changes even in small neurones.

This article outlines some of the theoretical and practical Cited by: intracellular ‘sharp’ microelectrode recording; intracellular microelectrodes, and advantages; impalement, and cell stress; sharp intracellular microelectrodes; electrode capacitance; microelectrode amplifiers, current through microelectrode; amplitude of changes in membrane, neuronal action potential.

Abstract. Ca 2+-sensitive microelectrodes generate a voltage proportional to the logarithm of the calcium activity (pCa) at their principle they are simple to understand and calibrate, but difficult to make and use intracellularly.

Their main advantages over other methods of measuring pCa i (such as fluorescent dyes and luminescent proteins) are that they measure pCa at one point and Cited by: 4. Intracellular pH (pH i) has been studied extensively for over a century using a wide range of techniques.

These techniques have been subject to constant improvements to the extent that useful measurements can now be made in even the smallest of cells. This chapter outlines the development of ion-sensitive microelectrodes and dyes and highlights. The resting pH of the rat lens was determined using H +-sensitive liquid membrane microelectrodes and found to be 689 when measured in a perifusing solution of pH 7 The pH of the rat vitreous humour was also measured and was found to be 7Cited by: Ion-sensitive microelectrodes (ISEs) have been used to measure intracellular [Mg2+] ([Mg2+]i) in cardiac muscle, although most measurements have tended to overestimate the value due to the poor selectivity of the Mg2+ ionophore in the sarcoplasm and to inaccurate collation of individual ISE measurements.

This paper highlights the correct method Cited by: 8. Additional Physical Format: Online version: Thomas, R.C. (Roger Christopher). Ion-sensitive intracellular microelectrodes. London ; New York: Academic Press, The Laboratory Computer: A Practical Guide for Physiologists and Neuroscientists introduces the reader to both the basic principles and the actual practice of recording physiological signals using the computer.

It describes the basic operation of the computer, the types of transducers used to measure physical quantities such as temperature and pressure, how these signals are amplified and. The microelectrode technique is today the most widely used method in electrophy- siology. Microelectrodes offer a unique approach to measurements of electrical pa- rameters and ion activities of single cells.

Several important breakthroughs in trans- port physiology have arisen from microelectrode studies. Undoubtedly, there is a progressively wide-spread use of conventional and ion-selective.

Thomas RC, Moody WJ () Ion-sensitive microelectrodes for intracellular use. Trends Biochem Sci –87 Google Scholar Thomas RC, Oehme M, Simon W () Lithium accumulation by snail neurones measured by a new Li + -sensitive by: Ion-sensitive intracellular microelectrodes: how to make and use them / R.

Thomas; Ion-selective microelectrodes, edited by Herbert J. Berman and Normand C. Hebert; The Application of ion-selective microelectrodes / editor, Thomas Zeuthen.

Ion-sensitive intracellular microelectrodes: how to make and use them / R. Thomas Ion-selective microelectrodes, edited by Herbert J. Berman and Normand C. Hebert The Application of ion-selective microelectrodes / editor, Thomas Zeuthen. Procedures for manufacturing double-barrelled ion-sensitive microelectrodes employing liquid sensors.

Journal of Biochemical and Biophysical Methods13 (1), DOI: /X(86) M. Desilets, C. by: Fozzard H. A., Chapman R. A., and Friedlander I. () Measurements of intracellular calcium ion activity with neutral exchanger ion sensitive microelectrodes. Cell Calcium 6.

This practical book provides the detailed methodology and expert guidance required for measuring and manipulating cytosolic ion concentrations. In addition, the strengths, weaknesses, and pitfalls of various techniques are presented.

It is an invaluable source for those needing an objective evaluation of current methodologies and for those contemplating setting up such procedures.A one-source.

Voipio, Juha; Pasternack, M ; MacLeod, K. / Ion-sensitive lectrode Techniques: The Plymouth Workshop Handbook. editor / D Ogden. ed Cited by: In this review, we describe a system in which we (a) microinject oocytes with cRNA that encodes a transporter fused to enhanced green fluorescent protein, (b) use fluorometry to assess the yield of the fusion protein in live oocytes, and (c) use ion-sensitive microelectrodes to assay the activity of expressed transporter by: An ion-sensitive microelectrode study on the effect of a high concentration of ivermectin on chloride balance in the somatic muscle bag cells of Ascaris suum - Volume Issue 4 - H.

Parri, M. Djamgoz, L. Holden-Dye, R. Walker. All animal cells are surrounded by a lipid bilayer membrane which separates the cytoplasm from the outside environment. The cell maintains an electrical membrane potential, negative inside, by active transport of ions membrane potential is a stored energy source which the cell can utilize to operate various molecular devices in the membrane 2.

Data of the intracellular electrolyte concentration of potassium and chloride in cultured muscle cells measured by x-ray analysis were compared by using the different activity coefficients with intracellular potassium and chloride activities measured with double-barrelled microelectrodes.

By using an activity coefficient of95% of the potassium microelectrode measurements are in Cited by: Determining the effective concentration (i.e., activity) of ions in and around living cells is important to our understanding of the contribution of those ions to cellular function.

Moreover, monitoring changes in ion activities in and around cells is informative about the actions of the transporters and/or channels operating in the cell membrane. The activity of an ion can be measured using a Cited by: Measurements of the intracellular activity of Ca (aiCa), Na (aiNa) and H (pHi) ions have been made with resin-filled ion-sensitive micro-electrodes in ferret ventricular trabeculae.

The mean values in quiescent muscle at 30 degrees C were: aiNa, +/- mM; aiCa, +/- nM, and pHi, +/- Cited by: Measurement of intracellular calcium ion activity with neutral exchanger ion sensitive microelectrodes. Fozzard HA, Chapman RA, Friedlander IR.

Micropipettes filled with the neutral liquid ion exchanger ETH can be used to make microelectrodes that are sensitive to cytoplasmic levels of Ca2+.Cited by:. The design and fabrication of an Ion-Selective Electrode (ISE) array platform for in-vitro intracellular recording are presented. The platform is composed of two parts: 1) a glass chip with.COVID Resources.

Reliable information about the coronavirus (COVID) is available from the World Health Organization (current situation, international travel).Numerous and frequently-updated resource results are available from this ’s WebJunction has pulled together information and resources to assist library staff as they consider how to handle coronavirus.Perspective: Ion-Selective Microelectrodes: Their Potential in the Study of Living Matter In Vivo Ion-Selective Electrode Response in Biologic Fluids Glass Microelectrodes for pH Some Problems with an Intracellular PO2 Electrode Some Problems with the Antimony Microelectrode --II.

Intracellular Applications